The intergenic small non-coding RNA ittA is required for optimal infectivity and tissue tropism in Borrelia burgdorferi.

The intergenic small non-coding RNA ittA is required for optimal infectivity and tissue tropism in Borrelia burgdorferi.

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Post-transcriptional regulation via small regulatory RNAs (sRNAs) has been implicated in diverse regulatory processes in bacteria, including virulence.One class of sRNAs, termed trans-acting sRNAs, can affect Short-term wind power combination forecasting method based on wind speed correction of numerical weather prediction the stability and/or the translational efficiency of regulated transcripts.In this study, we utilized a collaborative approach that employed data from infection with the Borrelia burgdorferi Tn library, coupled with Tn-seq, together with borrelial sRNA and total RNA transcriptomes, to identify an intergenic trans-acting sRNA, which we designate here as ittA for infectivity-associated and tissue-tropic sRNA locus A.

The genetic inactivation of ittA resulted in a significant attenuation in infectivity, with decreased spirochetal load in ear, heart, skin and joint tissues.In addition, the ittA mutant did not disseminate to peripheral skin sites or heart tissue, suggesting a role for Statistics of Smoke Sphericity and Optical Properties Using Spaceborne Lidar Measurements ittA in regulating a tissue-tropic response.RNA-Seq analysis determined that 19 transcripts were differentially expressed in the ittA mutant relative to its genetic parent, including vraA, bba66, ospD and oms28 (bba74).

Subsequent proteomic analyses also showed a significant decrease of OspD and Oms28 (BBA74) proteins.To our knowledge this is the first documented intergenic sRNA that alters the infectivity potential of B.burgdorferi.